Cetrimide agar is a selective medium for the isolation and detection of Pseudomonas aeruginosa from pharmaceutical, clinical and cosmetic samples. The formulation is complaint with the requirements of the Harmonised USP/EP/JP.

Detection is achieved using the unique ability of P. aeruginosa to produce the water soluble, bright green pigment pyocyanin. The production of this pigment is stimulated by the presence of magnesium chloride and di-potassium sulphate in the medium. The addition of glycerol (10ml/l) is required as this compound serves as an energy source. Cetrimide, a quaternary ammonium compound, is also present to suppress the growth of other Pseudomonas spp. as well as Gram-negative and Gram-positive organisms. Pancreatic digest of gelatin provides the required nitrogen, carbon and vitamins.

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