Dermatophyte Test Medium

KM0184

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Dehydrated Culture Media products are formulated to supply the required nutrients to allow for the growth of microorganisms.

Used in combination with a variety of selective agents and incubation conditions a wide range of specific organisms can be isolated.

With careful raw material selection of the various media components E&O can ensure a consistent level of quality and performance.

For each formulation the necessary ingredients are accurately weighed, combined and blended together to produce a homogenous powdered product.

Dermatophyte Test Medium

KM0184

A selective medium for the isolation and detection of dermatophytic fungi originally developed by Taplin et al.

Dermatophytes appear as fluffy colonies with reddening of the medium while other fungi cause the medium to become yellow due to acid production. Yeasts will also grow on this medium but are readily distinguished by their distinct white/creamy colonies and distinctive smell.

Soy peptone provides the required carbon, nitrogen and vitamins. Glucose is a fermentable carbohydrate. The inclusion of phenol red assists in the differentiation between saprophytic and environmental fungi. Although the low pH (pH 5.5) of the medium inhibits most bacteria chloramphenicol and cycloheximide are often added to further reduce the risk when processing material that may be more heavily contaminated.

NB: Prolonged incubation should be avoided as this may cause fungi other than dermatophytes to turn the medium red.

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Shelf Life
1095 days
Storage Temperature
10–30°C

pH

5.5 ± 0.2

Appearance

Fine Powder
Colour
Orange

Detailed performance specifications and Quality Control testing protocols are available in the IFU located in the supporting documentation section