LB Agar (Miller)

BM5280

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Dehydrated Culture Media products are formulated to supply the required nutrients to allow for the growth of microorganisms.

Used in combination with a variety of selective agents and incubation conditions a wide range of specific organisms can be isolated.

With careful raw material selection of the various media components E&O can ensure a consistent level of quality and performance.

For each formulation the necessary ingredients are accurately weighed, combined and blended together to produce a homogenous powdered product.

LB Agar (Miller)

BM5280

LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s. These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. Vitamins and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Tryptone is used to provide essential amino acids to the growing bacteria. LB Agar, Miller contains 10 g/L of sodium chloride, different from the levels in Lennox formulations. This allows the researcher to select the optimal salt concentration for a specific strain.

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Shelf Life
182 days
Storage Temperature
15–30°C

pH

7.0 ± 0.2

Appearance

Firm Gel
Colour
Straw

Detailed performance specifications and Quality Control testing protocols are available in the IFU located in the supporting documentation section