MRS Broth

KM0030

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Dehydrated Culture Media products are formulated to supply the required nutrients to allow for the growth of microorganisms.

Used in combination with a variety of selective agents and incubation conditions a wide range of specific organisms can be isolated.

With careful raw material selection of the various media components E&O can ensure a consistent level of quality and performance.

For each formulation the necessary ingredients are accurately weighed, combined and blended together to produce a homogenous powdered product.

MRS Broth

KM0030

This medium is intended for the cultivation and enumeration (via the Miles and Misra technique) of Lactobacillus spp. from a variety of sources and can be used in conjunction with MRS Agar (KM0080).

This medium is a modification on the formulation developed by de Man, Rogosa and Sharpe.(1)

The peptones, beef extract, yeast extract provides the required carbon, nitrogen and vitamins in this medium. Glucose is a fermentable carbohydrate. Potassium phosphate is a buffering agent. Selectivity of the medium is achieved through the use of

ammonium citrate and sodium acetate, inhibiting microorganisms such as streptococci and moulds. The magnesium sulphate, manganese sulphate and polysorbate 80 act as growth stimulants.

The medium can be made more specific for lactobacilli generally by lowering the pH to between 5.0 and 5.5.

This has the effect of inhibiting most streptococci that may otherwise grow on the medium and can be readily confused with lactobacilli.

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Shelf Life
1095 days
Storage Temperature
10–30°C

pH

6.5 ± 0.2

Appearance

Liquid
Colour
Dark straw

Detailed performance specifications and Quality Control testing protocols are available in the IFU located in the supporting documentation section