Edwardsiella ictaluri medium

KM0026

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Dehydrated Culture Media products are formulated to supply the required nutrients to allow for the growth of microorganisms.

Used in combination with a variety of selective agents and incubation conditions a wide range of specific organisms can be isolated.

With careful raw material selection of the various media components E&O can ensure a consistent level of quality and performance.

For each formulation the necessary ingredients are accurately weighed, combined and blended together to produce a homogenous powdered product.

Edwardsiella ictaluri medium

KM0026

Edwardsiella ictaluri medium is a selective medium for the isolation of Edwardsiella spp. based on the formulation by Shotts et al. (1) Edwardsiella spp. may be differentiated from other microorganisms on this medium due to its colony morphology.

The peptones provide the required carbon, nitrogen and vitamins. Sodium chloride maintains the osmotic balance. Colistin and bile salts are selective agents to inhibit most Gram-negative and Gram- positive organisms. Mannitol is a fermentable carbohydrate and bromthymol blue is a pH indicator. Phenylalanine and ferric citrate are used to detect phenylalanine deaminase activity in Proteus spp. Agar is a solidifying agent.

Shotts et al. (1) noted Proteus spp. swarming can overgrow on a mixed cultured sample. Therefore, a selective supplement (LS0021) may be added to the medium to restrict Proteus spp. swarming

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Shelf Life
1095 days
Storage Temperature
10–30°C

pH

7.1 ± 0.2

Appearance

Fine Powder
Colour
Straw

Detailed performance specifications and Quality Control testing protocols are available in the IFU located in the supporting documentation section